Quantitative ELISA Finder Feed Meat Urine Food Safety Test Kit

Principle and Application : This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Clenbuterol (CLE) in the sample such as urine, tissue and feed. The kit is composed of Microtiter Plate coated with coupled antigens, HRP conjugate, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the CLE in the samples will compete with the coupled antigens to combine with anti-CLE antibodies. After adding HRP conjugate, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with CLE content. Lastly, by comparing the obtained absorbance values with the standard curve, we can calculate the content of CLE content in the sample.